Paper I · 2 General Science & Technology · 2.11 Biotechnology

2.11.1 Recombinant DNA technology: restriction enzymes, vectors and PCR

Recombinant DNA technology cuts DNA with restriction enzymes, joins it to a vector with ligase, grows it in a host, and PCR makes millions of copies of a chosen DNA segment.

  • APPSC Paper I · Paper I
  • Standard level
  • 5 min read
  • Full course

Key terms

  • rDNA
  • restriction enzyme
  • EcoRI
  • ligase
  • plasmid
  • vector
  • Ti plasmid
  • PCR
  • Taq polymerase
  • gel electrophoresis
  • DNA fingerprinting
  • Southern blot

What this note covers

  1. Core factsFoundation
  2. ExplainedStandard
  3. DeeperAdvanced
  4. Mains writingMains writing
  5. Common trapsFoundation
  6. Quick revisionFoundation

Read the full note in the app. It is part of the APPSC Paper I full course in Medha AI. Free in the app: the first chapter of each topic.

Read the full note in the app General Science & Technology questions with answers

All Biotechnology notes · APPSC Paper I syllabus · APPSC Paper I Mock 1 with answers · Start free

Medha AI writes its own notes and checks them against the sources listed. Medha AI is an independent preparation service. It is not affiliated with, endorsed by or connected to APPSC, TSPSC, MPSC, UPSC or any other commission or government body.